DNA Coursework Example | Topics and Well Written Essays - 750 words

DNA - Coursework Example The enzyme, pyruvate dehydrogenase kinase in humans, has two domains ïÆ'   HATPase_c (Histidine kinase) and BCDHK_Adom3 (Mitochondrial branched-chain alpha-ketoacid dehydrogenase kinase). These domains are responsible for the functionality of the protein. The HATPase_c is an ATP binding domain. The enzyme inactivates pyruvate dehydrogenase using ATP. The HATPase_c domain is responsible for the enzyme’s kinase activity. The secondary structure of the pyruvate dehydrogenase kinase protein comprises of two sheets, two beta hairpins, two beta bulges, seven strands, fifteen helices, twenty-eight helix-helix interactions, twenty beta turns and two gamma turns. It also has a potassium ion ligand. The total alignment score for the three sequences is 99.0 for human and chimpanzee, 97.0 for human and dog, and 97.0 for chimpanzee and dog. Most of the amino acids are conserved in all the sequences, except for a few minor variations. The highest score for human and chimpanzee shows a high protein sequence similarity between the two. The entire stretch of the sequences with conserved amino acid clusters are highlighted below: Now that you have familiarized yourself with these sites, I would like you to make a small addition of your own initiative to the report by using any of the features described above or any other tool that you may have discovered. Using the sequence alignment of the pyruvate dehydrogenase kinase enzyme of human, chimpanzee and dog, created using the CLUSTALW program, a phylogram guide tree was created. This tree shows the phylogeny of the three